石灰添加量对平菇生长发育的影响

以平菇‘新831’为试材,研究了不同石灰添加量对棉籽壳熟料栽培平菇的影响。结果表明:在棉籽壳中添加石灰能够提高培养料pH值、减低杂菌感染率、降低菌丝生长速度、减弱菌丝长势、延迟出菇时间,添加适量石灰可以提高平菇栽培生物学效率。石灰添加量为2%时,平菇栽培生物学效率最高。     

龙葵毛状根诱导条件的研究

以发根农杆菌A4、C58C1和A1476为诱导菌株,探讨外植体类型、发根农杆菌种类、侵染时间、预培养时间、菌液浓度和共培养天数对龙葵毛状根诱导率的影响。结果表明:外植体以叶片诱导率最高;发根农杆菌A4、C58C1和A1476侵染龙葵叶片均能诱导出毛状根,C58C1诱导率较高;较佳侵染时间为5min;发根农杆菌浓度在OD600=0.6时诱导效果较好;预培养和共培养时间均为2d时诱导率较高。该试验为大量生产毛状根,并利用毛状根培养技术生产药物龙葵碱提供了试验基础。     

Effects of ursodeoxycholic acid on proinflammatory cytokines in children with infantile hepatitis syndrome

AIM: To observe the effect of ursodeoxycholic acid (UDCA) on the treatment of infantile hepatitis syndrome (HIS) and to investigate its mechanism. METHODS: The children with infantile hepatitis syndrome were divided into conventional treatment group and the UDCA treatment group. Twenty healthy children were selected as normal control. The children in conventional therapy group were given antiviral and hepatoprotective treatments. The children in UDCA treatment group were given ursodeoxycholic acid (10 mg·kg^-1·d^-1) in addition to the conventional treatment group for 2 to 3 weeks. The levels of total bilirubin (TBIL), direct bilirubin (DBIL), alanine aminotransferase (ALT), glutamyltransferase (GGT), total bile acids (TBA) and TNF-α, IL-6 were detected before admission and 2 weeks later.RESULTS: The levels of TNF-α and IL-6 were significantly higher in the children with IHS than those in the normal control (P<0.01). The levels of TBIL, DBIL, ALT, GGT, TBA, TNF-α and IL-6 in conventional treatment group were reduced after therapy (P<0.01). All the above index in UDCA treatment group were decreased compared with conventional treatment group (P<0.01). CONCLUSION: On the basis of conventional therapy, ursodeoxycholic acid effectively alleviates the systemic inflammatory response in the children with IHS, reduces the liver damages.     

Transplantation of bcl-2 gene-modified bone marrow mesenchymal stem cells improves cardiac function and angiogenesis in rabbit ischemic car-diac insufficiency model

AIM: To investigate the effects of transplantation of bone marrow mesenchymal stem cells (BMSCs) modified by bcl-2 gene on myocardial cell apoptosis, angiogenesis and cardiac function in the rabbit after acute myocardial infarction (MI).METHODS: The rabbit BMSCs were isolated, cultured and purified in vitro. The BMSCs were transfected with adenovirus or adenovirus-Bcl-2. The rabbit model of MI was established by ligation of left anterior descending branch. The rabbits were injected with Ad-Bcl-2-BMSCs (MI+Bcl-2-BMSCs group), Ad-BMSCs (MI+BMSCs group) and DMEM (MI group) in infarction marginal zone 2 weeks after ligation. The cardiac function was evaluated by echocardiography.The apoptosis of myocardial cells was measured by TUNEL. The mRNA expression of VEGF was detected by real-time PCR. The expression of CD31 was examined by immunohistochemical staining, and new blood capillaries were counted at 4 weeks after BMSCs transplantation. The correlation of the above values with cardiac function was analyzed.RESULTS: The cardiac function was better, the apoptotic rate was lower, the mRNA expression of VEGF and the capillary density were higher in both MI+Bcl-2-BMSCs group and the MI+BMSCs group than those in MI group, and those in MI+Bcl-2-BMSCs group increased more obviously.The left ventricular ejection fraction (LVEF) had a negative correlation with the myocardial cell apoptosis rate. A positive correlation with the mRNA expression level of VEGF and the capillary density was also observed.CONCLUSION: The transplantation of BMSCs modified by bcl-2 gene significantly reduces the myocardial cell apoptosis, promotes angiogenesis, improves heart function of the rabbits with MI.     

番茄果实有机酸总含量的QTL分析

以番茄高酸品种"12680"为母本,低酸品种"12574"为父本,配制杂交组合,构建含有260个单株的F2代群体为试材,采用酸碱滴定法测量果实的总酸含量,并进行遗传图谱构建及QTL分析,研究番茄果实中有机酸含量的遗传规律并找到与有机酸含量相关的QTL位点。结果表明:在256对AFLP引物和185对SSR引物中获得多态性良好的AFLP引物24对,SSR引物17对;构建连锁图谱,该图谱包括6个连锁群,共42个标记,覆盖基因组长度1 358.93cM,检测LOD值大于2.5的标记2个,贡献率分别为20.27%、10.98%,均分布在第2连锁群上。其中Ta1为负向加性效应,Ta2为正向加性效应。     

生姜提取液涂膜对鲜切苹果贮藏品质的动态变化

以"红富士"苹果为试材,采用无菌蒸馏水、1%海藻酸钠、0.5%海藻酸钠+0.1%生姜提取液、1%海藻酸钠+0.1%生姜提取液4个处理,PE保鲜袋4℃下贮藏,研究了生姜提取液及其可食性复合膜对鲜切"红富士"苹果的贮藏品质的影响。结果表明:海藻酸钠+生姜提取液处理在阻止水分蒸腾,维持硬度,降低褐变程度,减少营养物质的损耗,抑制多酚氧化酶(PPO)活性和丙二醛(MDA)的积累,减少微生物侵染方面,其综合效果要优于仅用海藻酸钠涂膜的处理及无菌蒸馏水处理的鲜切苹果。通过模糊数学感官评价方法对不同处理的鲜切苹果进行评判,得出最佳组合为1%海藻酸钠+0.1%生姜提取液。     

几份新选育香蕉种质资源的ISSR分析

利用ISSR标记技术对14份香蕉种质的遗传多态性进行了分析。从15个ISSR引物中筛选出12个多态性引物,共扩增出DNA条带126条,其中多态性条带101条,占80.2%。鉴定出4条特异DNA片段和特异缺失DNA片段7条。依据相似系数0.88的水平,将14份香蕉种质划分为4大类,与传统形态学分类结果相同。发现香牙蕉的遗传多样性较低,新选育的抗病突变型与母本之间不具有紧密的亲缘关系,不同香蕉种质对枯萎病的抗性强弱与其遗传相似性之间没有明确的相关性。     

丽江云杉家系苗期性状通径分析与优良家系初选

对91个丽江云杉(Picea likiangensis)2年生实生苗的苗高、地径、侧枝数、根系长和干重5个生长性状进行遗传分析和通径分析,并采用综合选择指数法对丽江云杉进行优良家系的评选。结果表明：2年生丽江云杉实生苗家系间各性状存在极显著差异(P＜0.01),其中侧枝数的遗传力最大,根系长的遗传力最小;性状的遗传变异系数干质量最大,苗高最小;地径对苗高的直接贡献最大,根系长通过地径对苗高的间接作用最大。通过综合选择指数法,评选出12个优良家系,这些家系的苗高实际增益为13.10%～29.58%,遗传增益为11.17%～25.24%。     

基于计算机视觉的全自动原木板材分检系统研究

原木板材的颜色纹理检测以及分类是木质产品生产过程中的一道重要工序。本文讨论分析了板材检测分类系统的硬件设计和系统涉及的算法，在分析不同聚类分析方法的基础上，提出了采用最大最小距离聚类法实现板材聚类，并对有关算法进行了软件设计和相关的实验研究，表明了算法的有效性。     

纤维增强定向成型材强化结构设计计算

纤维增强定向成型材是国内外人造板研究发展的一项创新,是一种可替代天然林木的复合材料.本文利用复合材料强度设计的方法,对其强化结构进行了设计计算,建立了纤维增强定向成型材强化结构模型.